本文件规定了一种半静态方法，用于确定污染物对幼蜗牛（通常是螺旋蜗牛）生长和存活的影响。使用测试基质（根据研究目的的人工或天然土壤）通过皮肤和消化途径暴露动物，其中添加了规定量的以下物质: -物质、混合物或制剂； -土壤（受污染或质量不明）或废料。 该试验考虑了试验物质、制剂、土壤或废料的可能变化，因为在28天的试验期内，每周都会制备和更新试验混合物。 除了半静态方法(可选)之外，还可以实现静态方法。该方法见附录A。 该方法不适用于空气/土壤分配系数大于1的物质，或25°C时蒸汽压超过300 Pa的物质。

This document specifies a semi-static method for determining the effects of contaminants on growth and survival of young snails, usually Helix aspersa aspersa Müller. The animals are exposed via the cutaneous and digestive route using a test substrate (artificial or natural soil according to the objective of the study) to which defined amounts of the following are added: - substances, mixtures or preparations; - soils (contaminated or of unknown quality) or waste materials. This test takes into account the possible changes in the test substance, preparation, soil or waste material because the test mixtures are prepared and renewed every week during the 28-day test period. A static method may be implemented in addition to the semi-static method (optional). This method is described in Annex A. This method does not apply to substances for which the air/soil partition coefficient is greater than one, or to substances with vapour pressure exceeding 300 Pa, at 25 °C.