BS PD ISO/TS 21569-5:2016规定了检测转基因食品中使用的DNA序列的程序 （GM）通过实时PCR（聚合酶链反应）对植物进行检测。该方法检测到78个碱基 配对Figwort花叶病毒34S启动子DNA序列的长片段。这是通用汽车公司的一部分 植物被指示为FMV启动子（P-FMV），在其他转基因植物中被指示为FMV增强子（E-FMV）。该方法已开发并验证用于分析从食品中提取的DNA。可能是的 也适用于分析其他产品，如饲料和种子。该程序需要 从测试样本中提取足够数量和质量的可扩增DNA。通过P-FMV元素特异性方法扩增的DNA序列可以在以下样本中检测到: 含有自然产生的菲格沃特花叶病毒的DNA。 因此，有必要进行确认 筛查结果呈阳性。需要使用特定构造或特定事件进行进一步分析 方法。交叉引用:ISO 21569ISO 21570ISO 21571:2005ISO 24276ISO/IEC 17025ISO 16577购买本文件时提供的所有当前修订版均包含在购买本文件中。

BS PD ISO/TS 21569-5:2016 specifies a procedure for the detection of a DNA sequence used in genetically modified (GM) plants by means of a real-time PCR (polymerase chain reaction). The method detects a 78 base pairs long segment of the Figwort mosaic virus 34S promoter DNA sequence. This segment in some GM plants is indicated as FMV promoter (P-FMV) and in other GM plants as FMV enhancer (E-FMV).The method was developed and validated for the analysis of DNA extracted from foodstuffs. It may be suitable also for analysis of other products such as feedstuffs and seeds. The procedure requires the extraction of an adequate quantity and quality of amplifiable DNA from the test sample.The DNA sequence amplified by the P-FMV element-specific method can be detected in samples which contain DNA of the naturally occurring Figwort mosaic virus. For this reason, it is necessary to confirm a positive screening result. Further analyses are required using construct-specific or event specific methods.Cross References:ISO 21569ISO 21570ISO 21571:2005ISO 24276ISO/IEC 17025ISO 16577All current amendments available at time of purchase are included with the purchase of this document.