1.1 这些测试方法包括当被测试的吸收垫用作保留细菌的营养蓄水池和介质供应源时，测定保留在膜过滤器上的生物体的营养保持能力和对细菌生长的毒性或营养影响。 1.2 所述试验在直径47 mm的圆盘上进行，尽管其他尺寸的圆盘可用于细菌培养技术。 1.3 以国际单位制表示的数值应视为标准值。本标准不包括其他计量单位。 1.4 本标准并非旨在解决与其使用相关的所有安全问题（如有）。 本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.5 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 这些试验方法适用于鉴定与膜过滤器一起使用的吸收垫，以进行细菌计数。 5.1.1 所述测试方法适用于供应商和用户对吸附垫的质量控制测试，以及细菌计数中与膜过滤器一起使用的吸附垫的规格测试。 5.2 其他纯培养生物及其适当的培养基可以替代 大肠杆菌 和M-FC介质，用于规范测试。

1.1 These test methods cover the determination of the nutrient-holding capacity and the toxic or nutritive effect on bacterial growth of organisms retained on a membrane filter, when the absorbent pad being tested is used as a nutrient reservoir and medium supply source for the retained bacteria. 1.2 The tests described are conducted on 47 mm diameter disks, although other size disks may be employed for bacterial culture techniques. 1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 These test methods are appropriate for qualifying absorbent pads used with membrane filters for bacteriological enumeration. 5.1.1 The test methods described are applicable to quality control testing of absorbent pads by the suppliers and users of these pads and to specification testing of absorbent pads intended for use with membrane filters in bacteriological enumeration. 5.2 Other pure culture organisms and their appropriate culture medium may be substituted for the E. coli and M-FC media for specification testing, as required.