1.1 这一做法涵盖了快速生长的有活力和可培养的植物的检测和计数 分枝杆菌属 （RGM），或非结核病 分枝杆菌属 （NTM）在含水金属加工液（MWF）中，使用标准微生物培养方法在高非分枝杆菌背景群体存在下进行。 1.2 检测限为菌落形成单位（CFU）/mL金属加工液。 1.3 这种做法涉及被归类为2级病原体的生物体的培养，应由受过培训的微生物学家在设备适当的设施中进行。微生物学家还应该能够区分 分枝杆菌属 从培养皿上的其他微生物菌落中提取，并能够确认 分枝杆菌属 采用抗酸染色法。 1.4 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的使用者有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.5 本国际标准是根据世界贸易组织技术性贸易壁垒委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 ===意义和用途====== 5.1 这种做法可以恢复和计数快速增长的可存活和可培养的非结核病 分枝杆菌属 ( M.immunoenum ， 龟分枝杆菌 ， 乳杆菌 ， 偶发分枝杆菌 和 耻垢分枝杆菌 )在金属加工流场样品中存在高革兰氏阴性背景群体的情况下。该群体主要由革兰氏阴性菌和真菌组成。金属加工液的分枝杆菌污染被认为与金属研磨机械师的超敏性肺炎（HP）有关。对这些生物体的检测和计数将有助于更好地了解职业健康- 相关问题和更好地评估抗微生物农药的功效。 5.2 活的和可培养的分枝杆菌密度的测量（指南 E1326 )，结合分枝杆菌总数（包括可培养活菌（VC）、不可培养活杆菌（VNC）和不可培养不活菌（NV）计数），通常是建立两者之间任何可能关系的第一步 分枝杆菌属 以及职业健康问题（例如HP）。 5.3 该实践可用于调查研究，以表征金属加工液现场样品的可行培养分枝杆菌种群密度。 5.4 本规程也适用于通过平板计数技术测定分枝杆菌存活率来确定金属加工液配方的分枝杆菌耐药性。 5.5 这种做法也可用于评估杀微生物剂对 分枝杆菌属 在金属加工液中。

1.1 This practice covers the detection and enumeration of viable and culturable rapidly growing Mycobacteria (RGM), or non-tuberculosis Mycobacteria (NTM) in aqueous metalworking fluids (MWF) in the presence of high non-mycobacterial background population using standard microbiological culture methods. 1.2 The detection limit is one colony forming unit (CFU)/mL metalworking fluid. 1.3 This practice involves culture of organisms classified as Level 2 pathogens, and should be undertaken by a trained microbiologist in an appropriately equipped facility. The microbiologist should also be capable of distinguishing the diverse colonies of Mycobacteria from other microorganism colonies on a Petri dish and capable of confirming Mycobacteria by acid-fast staining method. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 This practice allows for the recovery and enumeration of viable and culturable, non-tuberculosis, rapidly growing Mycobacteria ( M. immunogenum , M. chelonae , M. absessus , M. fortuitum , and M. smegmatis ) in the presence of high Gram-negative background populations in metalworking fluid field samples. This population is predominantly comprised of Gram-negative bacteria and fungi. Mycobacterial contamination of metalworking fluids has been putatively associated with hypersensitivity pneumonitis (HP) amongst metalgrinding machinists. The detection and enumeration of these organisms will aid in better understanding of occupational health-related problems and a better assessment of antimicrobial pesticide efficacy. 5.2 The measurement of viable and culturable mycobacterial densities (Guide E1326 ), combined with the total mycobacterial counts (including viable culturable (VC), viable nonculturable (VNC) and nonviable (NV) counts), is usually the first step in establishing any possible relationship between Mycobacteria and occupational health concerns (for example, HP). 5.3 The practice can be employed in survey studies to characterize the viable-culturable mycobacterial population densities of metalworking fluid field samples. 5.4 This practice is also applicable for establishing the mycobacterial resistance of metalworking fluid formulations by determining mycobacterium survival by means of plate count technique. 5.5 This practice can also be used to evaluate the relative efficacy of microbicides against Mycobacteria in metalworking fluids.