ISO 22959:2009规定了测定食用脂肪和油中多环芳烃(PAH)的高效液相色谱(HPLC)方法。 该方法已针对椰子油、橄榄油、葵花籽油和大豆油进行了验证，并且可能适用于其他油，具体取决于适当参数的确定。 多环芳烃的最低定量水平为0.1 μ g/kg。可以与基线噪声区分的每种PAH的最低可能量尚未确定。对于每种PAH，该方法的经验证浓度范围为0.1 μ g/kg至3.5 μ g/kg。对于（轻度）多环芳烃含量>3.5 μ g/kg的样品，可以进行稀释，使其含量在经验证的范围内。也可以调整校准曲线的范围。然而，尚未验证超过3.5 μ g/kg的范围。 可通过该方法测定的PAHs有:蒽、菲、荧蒽、芘、芘、苯并[a]蒽、苯并[e]芘、苯并[a]芘、苝、苯并[ghi]苝、蒽、二苯并[a，h]蒽、冠烯、茚并[1，2，3-cd]芘、苯并[a]荧蒽、苯并[b]荧蒽、苯并[k]荧蒽。

ISO 22959:2009 specifies a high performance liquid chromatographic (HPLC) procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible fats and oils. The method has been validated for coconut, olive, sunflower, and soybean oil, and is possibly applicable to other oils, dependent on the determination of appropriate parameters. The lowest level of quantification for the PAHs is 0,1 μg/kg. The lowest possible amount of each PAH which can be distinguished from the baseline noise has not been determined. The validated concentration range of the method is 0,1 μg/kg to 3,5 μg/kg for each individual PAH. For samples containing (light) PAH contents > 3,5 μg/kg, dilution to bring the contents into the validated range is possible. It is also possible to adjust the range of the calibration curves. However, ranges exceeding 3,5 μg/kg have not been validated. PAHs which can be determined by this method are: anthracene, phenanthrene, fluoranthene, pyrene, chrysene, benzo[a]anthracene, benzo[e]pyrene, benzo[a]pyrene, perylene, benzo[ghi]perylene, anthanthrene, dibenzo[a,h]anthracene, coronene, indeno[1,2,3-cd]pyrene, benzo[a]fluoranthene, benzo[b]fluoranthene, benzo[k]fluoranthene.