ISO 4833-2:20 13规定了用于计数在30℃需氧培养后能够在固体培养基表面生长和形成菌落的微生物的水平方法。该方法适用于: a)用于人类消费或动物饲料的产品； b)食品和饲料生产以及食品处理领域的环境样本。 ISO 4833-2:20 13适用于: 1)含有可能在总菌群中占显著比例的热敏性生物的产品(例如冷藏和冷冻食品、干燥食品、其他可能含有热敏性生物的食品中的嗜冷性生物)； 2)含有专性需氧细菌的产品，所述专性需氧细菌可能形成总菌群的显著比例(例如假单胞菌属)； 3)含有小颗粒的产品，所述小颗粒可证明难以与倾倒平板中的菌落区分；4)其强烈的颜色阻止在倾倒板中识别菌落的产品； 5)作为食品质量评估的一部分，需要区分不同类型菌落的产品。 除了手动铺板技术外，ISO 4833-2:20 13的附录还规定了螺旋平板的使用，螺旋平板是一种进行表面菌落计数的快速方法。 ISO 4833-2:20 13对某些发酵食品和动物饲料检查的适用性有限，其他培养基或培养条件可能更合适。然而，该方法可应用于此类产品，即使这些产品中的主要微生物可能未被有效检测。 对于某些基质，ISO 4833-2:20 13中描述的方法可以给出与使用ISO 4833中描述的方法获得的结果不同的结果？1.

ISO 4833-2:2013 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies on the surface of a solid medium after aerobic incubation at 30 °C. The method is applicable to: a) products intended for human consumption or for animal feed; b) environmental samples in the area of food and feed production and food handling. ISO 4833-2:2013 is applicable to: 1) products containing heat-sensitive organisms that are likely to form a significant proportion of the total flora (e.g. psychrotrophic organisms in chilled and frozen foods, dried foods, other foods that may contain heat-sensitive organisms); 2) products containing obligately aerobic bacteria that are likely to form a significant proportion of the total flora (e.g. Pseudomonas spp.); 3) products that contain small particles that can prove difficult to distinguish from colonies in a pour plate; 4) products whose intense colour prevents the recognition of colonies in a pour plate; 5) products for which distinction between different types of colony is required as part of the assessment of food quality. In addition to the manual spread plating technique, an annex to ISO 4833-2:2013 also specifies the use of a spiral plater, a rapid method of performing surface colony counts. The applicability of ISO 4833-2:2013 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in these products are not detected effectively. For some matrices, the method described in ISO 4833-2:2013 can give different results to those obtained using the method described in ISO 4833？1.