本文件规定了动物饲料中苯甲酸和山梨酸的测定方法 采用高效液相色谱-紫外检测法（HPLC-UV）。 该方法用于测定苯甲酸和山梨酸的活性 饲料添加剂、预混料、饲料材料和复合饲料中的物质以及饲料中的苯甲酸 水该方法测定这些有机酸及其盐的总可萃取浓度。 建议由用户确定每种有机酸的方法工作范围 这个标准。工作范围的下限取决于矩阵和干扰 遇到。建议工作范围在5 mg/l和100 mg/l之间。 该方法已在实验室间研究中成功进行了测试，浓度在0.02%至100%之间 9,0 %.交叉引用:EN ISO 3696ISO 3696ISO 6498EN ISO 6498ISO 5725-2ISO 6497EN ISO 6497购买本文件时提供的所有当前修订版均包含在购买本文件中。

This document specifies a method for the determination of benzoic acid and sorbic acid in animal feeding stuffs by high-performance liquid chromatography method with ultraviolet detection (HPLC-UV). The method is intended to be used for the determination of benzoic acid and sorbic acid as active substances in feed additives, premixtures, feed materials and compound feed and for benzoic acid in water. This method determines the total extractable concentration of these organic acids and their salts. It is advisable that the working range of the method is determined for each organic acid by the user of this standard. The lower limit of the working range depends on the matrix and the interferences encountered. It is advisable that a working range between 5 mg/l and 100 mg/l is accessible. The method was successfully tested in an inter-laboratory study in concentrations between 0,02 % up to 9,0 %.Cross References:EN ISO 3696ISO 3696ISO 6498EN ISO 6498ISO 5725-2ISO 6497EN ISO 6497All current amendments available at time of purchase are included with the purchase of this document.