本研究将标准平板计数的结果与用于配水和井水样品的吖啶橙（AO）和2-（对碘苯基）-3-（对硝基苯基）-5-苯基氯化四氮唑（INT）组合技术的结果进行了比较。为了克服膜滤器孔隙对染色细胞识别的干扰，作者使用凝胶剥离技术将细菌细胞从膜滤器转移到明胶基质中，然后进行计数。结果表明，AOINT技术的使用为测定水样中代谢活性细菌总数提供了一种快速、灵敏的方法，使其成为监测问题供水的标准电镀的有用辅助手段。 此外，使用AOINT技术可以在4小时内检测到供水污染。包括25个参考文献、表格和图表。

This study compared the results of standard plate counts with those of the combined acridine orange (AO) and 2-(p- iodophenyl)-3-(pnitrophenyl)-5-phenyl-tetrazolium chloride (INT), or AOINT, technique for samples of distribution water and well water. To overcome the interference of a membrane filter's pores with the identification of stained cells, the authors used a gel-stripping technique to transfer the bacterial cells from membrane filters to a gelatin matrix before counts were made. Results show that the use of the AOINT technique provides a rapid and sensitive method for the determination of total numbers of metabolically active bacteria in a water sample, making it a useful adjunct to standard plating for monitoring problematic water supplies. In addition, water supply contamination can be detected in 4 hours using the AOINT technique. Includes 25 references, tables, figure.