Colonization of microorganisms and resultant high populations often occur on or within protective encrustations or tubercles inside a distribution system. Formation of these encrustations is affected by a variety of physical, chemical, and biological activities within the pipes, which can be examined in situ by scanning electron microscopy (SEM). SEM photographs demonstrate very graphically the survival of microorganisms in the distribution system and their possible role in tubercle formation. Includes 13 references, table, figures.