ISO 7404-5:2009规定了在油浸抛光表面上用显微镜测定煤镜质体反射率的方法。这些方法适用于来自单一煤层的煤或覆盖整个低、中、高煤阶煤的混合煤。 镜质体反射率测量可用于表征混合物中的成分。可以采取措施校正混合物中每个组分的镜质体百分比，或确定混合物中组分的比例，尤其是当组分的镜质体含量不同时。这种方法需要分析员鉴定镜质体。 通过解释自动化系统的结果而获得的镜质体反射率测量不在ISO 7404-5:2009的范围内。

ISO 7404-5:2009 specifies the methods for determining the reflectance of vitrinite of coals microcopically on the polished surfaces, immersed in oil. The methods are applicable to coals from single seams or coal blends covering the whole range of low-, medium- and high-rank coal. Vitrinite reflectance measurements can be used to characterize the components within blends. Measures can be taken to correct for the vitrinite percentage within each of the components of the blend or to determine the proportion of components in a blend, particularly when the components have dissimilar vitrinite contents. This method necessitates the identification of vitrinite by the analyst. Reflectance measurements on vitrinite obtained by interpreting the results from an automated system, are outside the scope of ISO 7404-5:2009.