本文件规定了标准热循环仪和实时热循环仪的安装、维护、温度校准和温度性能测试的要求。它适用于检测微生物，以及使用基于聚合酶链反应（PCR）的方法在食物链中的任何其他应用。 本文件适用于食品检测，但也适用于使用热循环仪的其他领域（例如环境、人类健康、动物健康、法医检测）。

This document specifies requirements for the installation, maintenance, temperature calibration and temperature performance testing of standard thermal cyclers and real-time thermal cyclers. It is applicable to the detection of microorganisms as well as any other applications in the food chain using polymerase chain reaction (PCR)-based methods. This document has been established for food testing, but is also applicable to other domains using thermal cyclers (e.g. environmental, human health, animal health, forensic testing).