本欧洲标准介绍了一种通过微生物3板试验来描述在完全饲料和牛奶替代品中的抗生素泰乐菌素，维吉尼亚霉素，螺旋霉素，杆菌肽锌和阿伏苯菌素在亚添加剂水平下筛选的方法。 该方法的检测限为:奥泊他宁，泰乐菌素，螺旋霉素和维吉尼亚霉素为1 mg/kg，杆菌肽锌为5 mg/kg。其他（兽医）抗生素的存在可能会干扰该方法。 此外，高浓度的金属（Cu，Zn）可能会干扰。该方法应用作定性筛选方法。通过TLC进一步分析阳性结果;为了确认目的需要LC-MS [1]。 可以实现锌杆菌肽锌（3 mg/kg）的检测下限（见表2），但应首先通过内部验证建立

This European Standard presents a method describing the screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in complete feeding stuffs and milk replacers by a microbiological 3-plate test. The limit of detection of the method is 1 mg/kg for avoparcin, tylosin, spiramycin and virginiamycin, and 5 mg/kg for zinc bacitracin. The presence of other (veterinary) antibiotics may interfere with the method. Furthermore, high concentrations of metals (Cu, Zn) may interfere. The method should be used as a qualitative screening method. Positive results can be analysed further by TLC; for confirmatory purposes LC-MS is required [1]. A lower limit of detection for zinc bacitracin (3 mg/kg) is achievable (see Table 2), but should be established with an in house validation first.