Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis
食物链的微生物学 聚合酶链反应(PCR)用于检测食源性病原体 致病性小肠结肠炎耶尔森菌和假结核耶尔森菌的检测
BS PD CEN ISO/TS 18867:2015 specifies two horizontal methods for detection of the pathogenic
bioserotypes ofY. enterocoliticaand one for detection ofY. pseudotuberculosisby using real-time PCRbased
methods. The described methods allow for the detection of the two pathogens in enrichments
and allow the isolation of colonies.Y. pestis, the causative agent of bubonic and pneumonic plague
harbours a variant of the ail gene as well and will be detected by the same primer/probe set asY.
pseudotuberculosis. However,Y. pestisis normally not associated with food. This Technical Specification
is applicable to products for human consumption, animal feeding stuffs, and environmental samples.Cross References:ISO 6887-1ISO 10273ISO 20837ISO 20838ISO 22119ISO 22174ISO 11133ISO 7218All current amendments available at time of purchase are included with the purchase of this document.