迄今为止，大多数隐孢子虫灭活研究都是在实验室规模上进行的。 设计全尺寸反应器的问题在于Cts（消毒剂浓度 x暴露时间，基于10%的进水到达污水的时间） 间歇反应器不考虑系统液压。因此，直接量化 为了更好地评价隐孢子虫的灭活效果，最好对消毒性能进行评估。 替代方法包括使用生物（Kim等人，2002年）和非生物（Chiou等人，2002年） al.，1997年；Marinas等人，1997年、1999年；Baeza和Ducoste，2004）替代指标。 非生物指标尤其令人感兴趣，因为没有特殊的生物设施 这是需要的。可以使用代理进行直接量化，因为它已经需要 考虑到系统液压。非生物替代指标 用于化学消毒性能的是荧光染料聚苯乙烯微球。 Chiou等人（1997年）使用这些非生物微球来模拟 用臭氧消毒灭活贾第虫，由Marinas等人（1999）模仿 臭氧分批全量水消毒灭活隐孢子虫 最近，Baeza和Ducoste（2004年）对隐孢子虫进行了模拟 间歇反应器中的顺序消毒。所有这些研究都显示了良好的前景 使用微球模拟微生物失活的结果。然而，目前还没有研究表明这一点 在连续流动系统中使用具有顺序消毒的微球来模拟 隐孢子虫灭活。 因此，这项研究是为了评估这种非生物效应 连续流动顺序消毒过程中的方法。包括19个参考文献、表格和图表。

To date, most Cryptosporidium inactivation studies have been done at the bench scale. The problem with designing full-scale reactors is that the Cts (disinfectant concentration x exposure time, based on time that 10% of influent water reaches the effluent) from batch reactors do not account for the system hydraulics. As a result, direct quantification of disinfection performance is preferred to better evaluate Cryptosporidium inactivation. Alternatives include the use of biological (Kim et al., 2002) and non-biological (Chiou et al., 1997; Marinas et al., 1997, 1999; Baeza and Ducoste, 2004) surrogate indicators. Non-biological indicators are of particular interest, since no special biological facilities are needed. Direct quantification can be made with the surrogate, since it already takes into account system hydraulics. The non-biological surrogate indicators that have been used for chemical disinfection performance are fluorescent dye polystyrene microspheres. These non-biological microspheres have been used by Chiou et al. (1997) to mimic Giardia inactivation with ozone disinfection, by Marinas et al. (1999) to mimic Cryptosporidium inactivation with ozone disinfection in batch and full-scale water treatment plants, and recently by Baeza and Ducoste (2004) to mimic Cryptosporidium sequential disinfection in batch reactors. All of these studies have shown promising results in using microspheres to mimic microbial inactivation. However, no study has used microspheres with sequential disinfection in a continuous flow system to mimic Cryptosporidium inactivation. Hence, this study was performed to evaluate this nonbiological approach in continuous-flow sequential disinfection processes. Includes 19 references, tables, figure.