在美国，紫外线（UV）反应器目前使用大肠杆菌噬菌体MS2作为挑战微生物进行验证； 然而，MS2明显比微小隐孢子虫更耐紫外线。目标 这项研究的目的是描述更好地匹配 隐孢子虫的剂量反应比MS2强。总体方法是选择 获取通过彻底的文献检索确定的潜在替代微生物，并 对潜在替代物进行台架试验，以确定其对药物的剂量反应 紫外线照射。在这些候选生物体中，只有那些表现出剂量反应的生物体 与C.parvum相似的是进一步的特征。微生物最大化的方法 大规模繁殖的产量（病毒替代物的一个必要特征）已经开发出来 并用于进一步筛选所选生物体的潜在用途。 基于这些 与隐孢子虫的反应最为相似 选择进行全面测试。包括数字。

In the U.S., ultraviolet (UV) reactors are currently validated using coliphage MS2 as the challenge microbe; however, MS2 is significantly more UV-resistant than Cryptosporidium parvum. The objective of this study was to characterize the UV dose response of microorganisms that better match the dose response of Cryptosporidium than does MS2. The overall approach was to select and acquire potential surrogate microorganisms identified from a thorough literature search and perform bench-scale testing of the prospective surrogates to determine their dose responses to UV irradiation. Of these candidate organisms, only those that demonstrated dose responses similar to C. parvum were further characterized. Methodologies to maximize microorganism yield for large scale propagation (a required characteristic of the virus surrogate) were developed and used to further screen the potential usefulness of the selected organisms. Based on these findings, the surrogate(s) that most closely resembled Cryptosporidium's response to UV was selected for full-scale testing. Includes figure.