本研究的目的是证明核糖体RNA靶向技术 可用于在确定消毒效果时量化活菌。这个 作者开发了一种新技术，可以同时测量核糖体RNA和DNA 前体核糖体RNA，核糖体生产中的关键中间体。核糖体RNA 在不动杆菌培养物中同时测定前体核糖体RNA calcoaceticus^T和粘生分枝杆菌。结果表明，前体核糖体 RNA是衡量生存能力的敏感指标。作者认为这种方法具有巨大的实用价值 利用分子生物学验证微生物消毒效果的承诺- 基于 监控。未来，结合分子生物学技术的快速发展 通过检测核糖体的生成，对细胞活力进行关键测量，可能会改变 消毒剂的评估方法。包括12个参考文献、图表。

The objective of this study was to demonstrate that ribosomal RNA-targeted techniques could be used to quantify viable microorganisms when determining disinfection efficacy. The authors have developed a new technique to simultaneously measure ribosomal RNA as well as precursor ribosomal RNA, a key intermediate in the production of ribosomes. Ribosomal RNA and precursor ribosomal RNA were measured simultaneously in cultures of Acinetobacter calcoaceticus^T and Mycobacterium mucogenicum. The results demonstrate that precursor ribosomal RNA is a sensitive measure of viability. The authors believe this approach holds tremendous promise for verifying disinfection efficacy for microorganisms using molecular biology-based monitoring. In the future, coupling the rapid developments in molecular biology-based technology with the critical measurement of cellular viability via detection of ribosome genesis may change the way disinfectants are evaluated. Includes 12 references, figures.