本文件规定了一种使用内标或外标测定佛手柑精油中bergapten含量的高效液相色谱（HPLC）方法。佛手柑（Risso et Poit。）怀特和阿恩。ex Engl.】，在柠檬精油【Citrus limon（L.）Burm.f.】，在苦橙精油（Citrus bigaradia Risso）和酸橙精油【Citrus aurantiifolia（Christm。）Swingle和Citrus latifolia Tanaka】中，所有这些都在bergapten中完全或部分减少。

This document specifies a high-performance liquid chromatographic (HPLC) method, using either an internal standard or external standard, for the determination of the bergapten content in essential oil of bergamot [Citrus aurantium ssp. bergamia (Risso et Poit.) Wight et Arn. ex Engl.], in essential oil of lemon [Citrus limon (L.) Burm. f.], in essential oil of bitter orange (Citrus bigaradia Risso) and in essential oil of lime [Citrus aurantiifolia (Christm.) Swingle and Citrus latifolia Tanaka], all of them fully or partially reduced in bergapten.