ISO 17289:2014规定了使用基于荧光猝灭的传感器测定水中溶解氧的光学方法。 测量可以是氧浓度（以毫克/升为单位）、饱和度百分比（溶解氧%）或两者兼而有之。根据使用的仪器，可根据制造商手册达到0.1 mg/l或0.2 mg/l的检测限。大多数仪器允许测量高于100%的值，即过饱和度。 注:当氧的分压高于空气中的分压时，过饱和是可能的。特别是在藻类生长旺盛的情况下，过饱和度可能高达200%甚至更多。 如果测量饱和度高于100%的水，则必须做出安排，防止在处理和测量样品期间氧气脱气。 同样，如果饱和度低于100%，防止氧气进入样品也很重要。 该方法适用于现场测量、溶解氧连续监测以及实验室测量。它是高颜色和浑浊水的首选方法之一，也适用于分析不适合温克勒滴定法的水，因为铁和碘固定物质可能会干扰ISO 5813中规定的碘量法。 该方法适用于饮用水、天然水、废水和盐水。如果用于海水或河口水域等含盐水域，则盐度校正对于氧气浓度测量至关重要。

ISO 17289:2014 specifies an optical method for the determination of dissolved oxygen in water using a sensor working on the basis of fluorescence quenching. Measurement can be made either as a concentration of oxygen in milligrams per litre, percentage saturation (% dissolved oxygen), or both. Depending on the instrument used, detection limits of 0,1 mg/l or 0,2 mg/l can be reached according to the manufacturer's manual. Most instruments permit measurement of values higher than 100 %, i.e. supersaturation. NOTE Supersaturation is possible when the partial pressure of oxygen is higher than in air. Especially in case of strong algae growth, supersaturation up to 200 % and more is possible. If waters with a saturation higher than 100 % are measured, it is essential to make arrangements to prevent the outgassing of oxygen during the handling and measurement of the sample. Similarly, it is important that the transport of oxygen into the sample is prevented if the saturation is below 100 %. The method is suitable for measurements made in the field and for continuous monitoring of dissolved oxygen as well as measurements made in the laboratory. It is one of the preferred methods for highly coloured and turbid waters, and also for analysis of waters not suitable for the Winkler titration method because of iron- and iodine-fixing substances, which can interfere in the iodometric method specified in ISO 5813. The method is suitable for drinking waters, natural waters, waste waters, and saline waters. If used for saline waters such as sea or estuarine waters, a correction for salinity is essential for concentration measurement of oxygen.