本国际标准规定了一种评估试验材料对植物生长的潜在毒性的方法 活性污泥中存在的好氧细菌。抑制作用仅限于那些能够 在选择的有机试验培养基上生长。 该方法提供了在长达6小时的潜伏期内对微生物产生抑制作用的信息。 本方法适用于水、废水和化学物质在以下条件下可溶: 测试。需要特别注意挥发性或有色材料以及形成浑浊悬浮液或悬浮液的材料 分散。 注1:挥发性试验材料的结果应谨慎解释，并可能低估任何抑制作用 由于难以维持试验瓶中的初始浓度，因此产生了影响。 注2:可测试形成浑浊悬浮液或分散液的有色材料和低水溶性材料 在许多情况下，使用颜色/浊度控制作为空白（见9.4）。 注3:本试验不适用的试验材料对活性污泥微生物的抑制作用可以 通过使用基于呼吸计量测量的抑制试验确定（见ISO 8192）。

This International Standard specifies a method for assessing the potential toxicity of a test material to the growth of aerobic bacteria present in activated sludge. The inhibitory effect is restricted to those microorganisms capable of growth on the chosen organic test medium. This method gives information on inhibitory effects on the microorganisms over incubation periods up to 6 h. This method is applicable to water, wastewater and chemical substances which are soluble under the conditions of the test. Special care is needed with volatile or coloured materials and materials which form turbid suspensions or dispersions. NOTE 1 Results with volatile test material should be interpreted with caution and are likely to underestimate any inhibitory effect because of the difficulties in maintaining the initial concentration in the test flasks. NOTE 2 Coloured materials and materials of low water-solubility which form turbid suspensions or dispersions can be tested in many cases using colour/turbidity controls as blanks (see 9.4). NOTE 3 Inhibitory effects on activated sludge microorganisms of test materials for which this test is not applicable can be determined by using an inhibition test based on respirometric measurements (see ISO 8192).