本文件规定了一种实时聚合酶链反应（real-time PCR）方法用于 从食物和饲料中提取驴特异性DNA的定性检测。它需要提取一个 从相关基质中提取足够数量的PCR扩增DNA，并可用于检测 从驴子（马驹）、骡子（马驹）中提取的驴子材料♂ ×亚洲马驹♀) 和 辛尼（马驹）♀ ×亚洲马驹♂). 该检测还检测斑马（Equius burchellii）。购买本文件时可获得的所有当前修订均包含在购买本文件中。

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of donkey-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of donkey material derived from donkey (Equus asinus), mule (Equus caballus♂ ×Equus asinus♀) and hinny (Equus caballus♀ ×Equus asinus♂). The assay also detects the species zebra (Equus burchellii).All current amendments available at time of purchase are included with the purchase of this document.