开曼水务局（WAC）向格兰德岛提供饮用水 开曼群岛。WAC水系统由两个反渗透（RO）装置和 位于红门路的抽水设施能够生产总计210万美元的水 加仑/天。该发电厂与三个水库相连，并带有一个联合水库 容量为300万美国加仑。第三个反渗透工厂位于下谷， 乔治镇以东大约10英里。这座工厂和抽水设施是 能够每天生产80万美国加仑，并与两个仓库相连 水库的总存储容量为300万美国加仑。水是生产出来的 通过反渗透从约250口深井中抽取含盐地下水，通过 一系列预过滤器，在通过一系列反渗透膜循环之前清除淤泥。 两个工厂的给水含有约1-2 mg/L H₂S。产品水 通过逆流脱气器，用酸处理，从而降低 pH值为6.0或更低。水用65%次氯酸钙消毒，正磷酸锌消毒 添加氢氧化钠以抑制腐蚀，添加氢氧化钠以控制pH值。第一阶段 四年的研究旨在描述WAC水的细菌生态 为了了解从入口到出口的配电系统之间的关系 生物膜和浮游生物。一项比较介质研究发现，R2A在26°C时 是更适合HPC生长的培养基，尽管没有显著差异 从R2A和mHPC获得的计数差异；给水生物在土壤上生长最好 在35°C下改良mHPC培养基。治疗后HPC计数较低（<6/mL），但 表观荧光直接计数显示，存在的细菌水平要高出数对数。 几十个菌落已经进行了继代培养，目前正在进行识别主要细菌的工作 群体，尤其是在整个系统中发现的群体。水温是一个关键因素 WAC配送系统细菌生态的重要因素，最大 温度从冬季（11月至4月）的24-27°C到夏季的28-33°C不等 （5月至10月）。这种设置的独特之处在于使用塑料或PVC管道材料 整个系统。正在分析管道破裂的样本，以比较 管道中的生物膜与水相中的细菌相连。所选样本的DNA特征 隔离正在进行中。这项研究是第一次全面的微生物学研究 淡化水系统的特征。包括14个参考文献、表格和图表。

The Water Authority-Cayman (WAC) supplies drinking water to the island of Grand Cayman. The WAC water system consists of two reverse osmosis (RO) plants and pumping facilities at Red Gate Road capable of producing a total of 2.1 million US gallons/day. The plant is linked to three storage reservoirs with a combined storage capacity of 3 million US gallons. A third RO plant is located at Lower Valley, approximately 10 miles east of George Town. This plant and pumping facility are capable of producing 0.8 million US gallons/day and are linked to two storage reservoirs with a combined storage capacity of 3 million US gallons. Water is produced from plants via RO, by pumping saline groundwater from ~250 deep wells through a series of prefilters to remove silt before circulation through a series of RO membranes. The feed water to both plants contains approximately 1-2 mg/L H₂S. The product water is passed through a counter flow degasifier, is treated with acid thereby reducing the pH to 6.0 or less. Water is disinfected with 65% Ca hypochlorite, Zn orthophosphate is added to inhibit corrosion and NaOH is added to control the pH. The first phase of this four-year study is designed to characterize the bacterial ecology of the WAC water distribution system from inlet to outlet in order to understand the relationship between biofilms and planktonic organisms. A comparative media study found that R2A at 26°C is the more appropriate media for growth of HPC although there was not a significant difference in counts obtained from R2A and mHPC; feedwater organisms grow best on modified mHPC media at 35°C. The HPC counts after treatment are low (<6/mL), but epifluorescence direct counts show several log greater levels of bacteria present. Several dozen colonies have been subcultured and work is underway to identify major groups, particularly those found throughout the system. Water temperature is an important factor in the bacterial ecology of the WAC distribution system, with maximum temperatures varying from 24-27°C in winter (November-April) to 28-33°C in summer (May to October). Unique to this setting is the use of plastic or PVC piping material throughout the system. Samples from pipe breaks are being analyzed to compare the biofilms in the piping to bacteria in the water phase. DNA characterization of selected isolates is underway. This study represents the first comprehensive microbiological characterization of a desal water system. Includes 14 references, tables, figures.