1.1 本规程规定了获得用于计算荧光样品颜色的精确双光谱光度数据所需的仪器测量要求、校准程序和材料标准。 1.2 本规程列出了特定方法、规程或规范中要求进行双谱光度测量时必须指定的参数。 1.3 这一实践特别适用于双光谱计，它产生光度定量的双光谱数据作为输出，有助于表征外观，而荧光光谱计则产生仪器相关的双光谱光度数据作为输出。 1.4 本实践的范围仅限于讨论反射几何条件下的物体颜色测量；它不包括在透射几何条件下对试样进行类似表征的规定。 1.5 本标准可能涉及危险材料、操作和设备。本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的使用者有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.6 本国际标准是根据世界贸易组织技术性贸易壁垒委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 ====意义和用途====== 5.1 双谱或双单色仪法是测定荧光样品一般（与光源无关）辐射转移特性的决定性方法 ( 2. ) .唐纳森辐射系数是一个工具- 以及样品与光源无关的光度特性，并可用于计算任何所需光源和观察者的颜色。这种方法的优点是，它提供了样品辐射传输特性的全面表征，而没有与源模拟和各种近似方法相关的不准确度。 5.2 该实践提供了一种用于选择用于提供所需精度的数据的双电子计的操作参数的程序。它还提供了通过材料标准进行仪器校准，以及选择合适的样品以获得测量精度。

1.1 This practice addresses the instrumental measurement requirements, calibration procedures, and material standards needed for obtaining precise bispectral photometric data for computing the colors of fluorescent specimens. 1.2 This practice lists the parameters that must be specified when bispectral photometric measurements are required in specific methods, practices, or specifications. 1.3 This practice applies specifically to bispectrometers, which produce photometrically quantitative bispectral data as output, useful for the characterization of appearance, as opposed to spectrofluorimeters, which produce instrument-dependent bispectral photometric data as output, useful for the purpose of chemical analysis. 1.4 The scope of this practice is limited to the discussion of object-color measurement under reflection geometries; it does not include provisions for the analogous characterization of specimens under transmission geometries. 1.5 This standard may involve hazardous materials, operations, and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 The bispectral or two-monochromator method is the definitive method for the determination of the general (illuminant-independent) radiation-transfer properties of fluorescent specimens ( 2 ) . The Donaldson radiance factor is an instrument- and illuminant-independent photometric property of the specimen, and can be used to calculate its color for any desired illuminant and observer. The advantage of this method is that it provides a comprehensive characterization of the specimen’s radiation-transfer properties, without the inaccuracies associated with source simulation and various methods of approximation. 5.2 This practice provides a procedure for selecting the operating parameters of bispectrometers used for providing data of the desired precision. It also provides for instrument calibration by means of material standards, and for selection of suitable specimens for obtaining precision in the measurements.