Animal and vegetable fats and oils. Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS-Method using slow alkaline transesterification and measurement for 2-MCPD, 3-MCPD and glycidol
动植物脂肪和油 GC/MS法测定脂肪酸结合的氯丙二醇(MCPD)和缩水甘油 缓慢碱性酯交换法和2-MCPD、3-MCPD和缩水甘油的测定
发布日期:
2018-10-11
BS EN ISO 18363-2:2018规定了平行测定甘醇以及油脂中以结合或游离形式存在的2-MCPD和3-MCPD的程序。该方法基于碱性催化酯裂解、释放的缩水甘油转化为单溴丙二醇(MBPD)以及衍生的游离二醇(MCPD和MBPD)与苯硼酸(PBA)的衍生化。虽然游离MCPD和缩水甘油在脂肪和油中的含量应该很低,甚至可以忽略不计,但如果存在游离分析物,它们将对结果产生成比例的影响。结果总是单个分析物的自由态和束缚态之和。该方法适用于固体和液体脂肪和油。本文件也适用于动物脂肪和用过的煎炸油和脂肪,但在分析这些基质之前要进行验证研究。牛奶和奶制品(或来自牛奶和奶制品的脂肪)不在本文件范围内。
交叉引用:ISO 3696ISO 18363-1ISO 5555ISO 18363-3购买本文件时可获得的所有现行修订均包含在购买本文件中。
BS EN ISO 18363-2:2018 specifies a procedure for the parallel determination of glycidol together with 2-MCPD and 3-MCPD present in bound or free form in oils and fats. The method is based on alkaline-catalysed ester cleavage, transformation of the released glycidol into monobromopropanediol (MBPD) and derivatisation of the derived free diols (MCPD and MBPD) with phenylboronic acid (PBA). Though free MCPD and glycidol are supposed to be present in fats and oils in low to negligible quantities only, in the event that free analytes are present, they would contribute proportionately to the results. The results always being the sum of the free and the bound form of a single analyte.This method is applicable to solid and liquid fats and oils. This document can also apply to animal fats and used frying oils and fats, but a validation study is undertaken before the analysis of these matrices.Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.Cross References:ISO 3696ISO 18363-1ISO 5555ISO 18363-3All current amendments available at time of purchase are included with the purchase of this document.