本powerpoint演示的目的是评估隐孢子虫的依赖性 去除进水中的卵囊和贾第鞭毛虫囊肿 卵囊和囊肿的浓度。抽样方法包括: 抓取取样 （10升样品， 浓缩至200毫升 与CSCC合作）； 连续采样 （CSCC直接连接 过滤废水30小时 运行时）；和 CSCC浓缩288升 过滤废水至200ml。威斯康星州的分析方法 卫生实验室 （WSLH）包括: 离心； 荧光抗体 染色； 流式细胞术用于 将微生物与 样品中的其他颗粒；和 放置分离的微生物 在幻灯片上按 显微镜检查。马什菲尔德诊所的分析方法 研究基金会 （MCRF）包括: 膜过滤； 荧光抗体 染色；和 在显微镜下计数。潜在影响包括: 使用常规地表水的供水设施 治疗可能无法达到卵囊水平 以及他们认为是的囊肿切除术 实现； 当检测到时，美国水源水中的典型计数为 大约100个微生物/L或更少；和 门多塔湖的外推结果达到了这个水平 建议删除可能仅为0.5 log到1.0 log，而不是 从2.5 log到3.0 log。包括桌子。

The objective of this powerpoint presentation was to evaluate the dependence of Cryptosporidium oocyst and Giardia cyst removal on the influent concentration of oocysts and cysts. The sampling approach included: Grab Sampling (10 L samples, concentrated to 200 mL with CSCC); continuous sampling (CSCC hooked up directly to filter effluent for 30 hour run time); and, CSCC concentrated 288 L of filter effluent to 200 mL. The analytical approach at Wisconsin State Laboratory of Hygiene (WSLH) included: centrifugation; fluorescent antibody staining; flow cytometry used to separate microbes from other particles in sample; and, separated microbes placed on slide and counted by microscopy. The analytical approach at Marshfield Clinic Research Foundation (MCRF) included: membrane filtration; fluorescent antibody staining; and, counting under a microscope. Potential implications include: water utilities using conventional surface water treatment may not be achieving the level of oocyst and cyst removal that they think they are achieving; when detected, typical counts in U.S. source waters are on the order of 100 microbes/L or less; and, extrapolation of Lake Mendota results to this level suggests removal may only be 0.5 log to 1.0 log, rather than 2.5 log to 3.0 log. Includes table.