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Standard Test Method for Using Seeded-Agar for the Screening Assessment of Antimicrobial Activity In Carpets 地毯中抗菌活性筛选评估用种子琼脂的标准试验方法
发布日期: 2023-06-01
1.1 该测试方法旨在(定性)评估地毯中或地毯上是否存在抗菌活性。使用该测试方法对抗菌和抗真菌活性进行定性评估。 1.2 使用半强度(营养和琼脂)胰蛋白酶大豆琼脂作为细菌的接种载体,半强度马铃薯葡萄糖琼脂作为霉菌分生孢子的接种载体。使用半强度琼脂可能会减少由于过量有机负荷而导致的抗微生物剂的过度中和。 1.3 该测试方法同时评估(视觉和立体显微镜)地毯纤维层和主要背衬层的抗菌活性。 1.4 使用此测试方法来评估新地毯以及反复清洗或暴露在使用条件下的地毯的抗菌处理的耐久性。 1.5 实践该试验方法需要掌握微生物技术知识。 1.6 本标准并非旨在解决与其使用相关的所有安全问题(如有)。本标准的使用者有责任在使用前制定适当的安全、健康和环境实践,并确定监管限制的适用性。 1.7 本国际标准是根据世界贸易组织技术性贸易壁垒委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 ====意义和用途====== 5.1 该测试方法提供了位于地毯表面纤维中或其上或结合到地毯背衬结构中(或两者)的抗微生物处理的快速筛选。 5.2 该测试方法模拟了地毯上可能出现的实际使用条件(例如,食物和饮料溢出、行人交通造成的污染、长期暴露在潮湿环境中)。 5.3 该测试方法提供了一种在有机负载条件下筛选抗微生物处理的活性和耐久性的方法。 5.4 该测试方法可同时评估多种地毯成分的抗菌活性。 5.5 在使用该测试方法进行测试之前,可对地毯进行清洁,以评估抗菌效果的耐久性。
1.1 This test method is designed to evaluate (qualitatively) the presence of antimicrobial activity in or on carpets. Use this test method to qualitatively evaluate both antibacterial and antifungal activity. 1.2 Use half strength (nutrient and agar) tryptic soy agar as the inoculum vehicle for bacteria and half strength potato dextrose agar as the inoculum vehicle for mold conidia. Use of half strength agars may reduce undue neutralization of an antimicrobial due to excessive organic load. 1.3 This test method simultaneously evaluates (both visual and stereo-microscopic) antimicrobial activity both at the fiber layer and at the primary backing layer of carpet. 1.4 Use this test method to assess the durability of the antimicrobial treatments on new carpets, and on those repeatedly shampooed or exposed to in-use conditions. 1.5 Knowledge of microbiological techniques is required for the practice of this test method. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 This test method provides for rapid screening of antimicrobial treatments located in or on the carpet face fiber or incorporated into the backing structure of the carpet (or both). 5.2 This test method simulates actual use conditions that may occur on carpets (for example, food and beverage spills, soiling from foot traffic, prolonged moisture exposure). 5.3 This test method provides a means to screen for activity and durability of an antimicrobial treatment under conditions of organic loading. 5.4 This test method provides for the simultaneous assessment of multiple carpet components for antimicrobial activity. 5.5 Carpets may be cleaned prior to testing with this test method in order to assess the durability of the antimicrobial effect.
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归口单位: E35.15
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