本文件规定了一种检测海洋和溯河鱼类中常见的异尖线虫科L3幼虫的方法。该方法适用于新鲜鱼和/或冷冻鱼，以及轻度加工的鱼制品，如腌制、腌制或冷熏。 该方法适用于通过估计鱼类肌肉组织中寄生虫的数量来量化寄生虫感染。 该方法不适用于确定检测到的寄生虫的种类或基因型。通过形态学和/或分子方法进行最终鉴定。

This document specifies a method for the detection of Anisakidae？L3 larvae commonly found in marine and anadromous fishes. The method is applicable to fresh fish and/or frozen fish, as well as lightly processed fish products, such as marinated, salted or cold smoked. This method is applicable to quantifying parasitic infections by estimating the number of parasites in the fish musculature. This method does not apply to determining the species or genotype of detected parasites. Final identification is made by morphological and/or molecular methods.