Microbiology of the food chain — Detection of Trichinella larvae in meat by artificial digestion method
食物链的微生物学 - 人工消化法检测肉类中的旋毛虫幼虫
发布日期:
2015-09-11
ISO 18743:2015规定了用于人类消费的单个动物尸体的肉中旋毛虫属的检测方法。肌肉阶段幼虫。它适用于检查来自家畜和森林动物物种的肉,这些动物可以被旋毛虫属的线虫感染。
该方法不允许鉴定检测到的寄生虫的物种或基因型;物种或基因型鉴定可以通过分子方法进行。
本国际标准中描述的方法旨在与OIE诊断试验和疫苗手册中的指南以及国际旋毛虫病委员会(ICT)用于旋毛虫试验和供人食用的尸体检查,除非已通过其他方式证明动物没有暴露于旋毛虫的风险。人工消化/磁力搅拌器法被认为是标准方法,因为它已被证明在验证研究中给出了最可靠的结果。
注:如果可以记录与本国际标准中描述的方法的等效性,则可以使用替代方法进行分析。
ISO 18743:2015 specifies a method of detection of Trichinella spp. muscle stage larvae in meat of individual animal carcasses intended for human consumption. It is applicable to the examination of meat from domestic and sylvatic animal species, which can be infected by nematodes of the genus Trichinella.
This method does not allow the identification of the species or genotype of detected parasites; species or genotype identification can be carried out by molecular methods.
The method described in this International Standard is intended to be used in conjunction with the guidelines in the OIE Manual of Diagnostic Tests and Vaccines and by the International Commission on Trichinellosis (ICT) for Trichinella testing and the inspection of carcasses intended for human consumption, unless it has been demonstrated by other means that the animal was not at risk for exposure to Trichinella.
The artificial digestion/magnetic stirrer method is considered to be the standard method because it has proven to give the most reliable results in validation studies.
NOTE Provided equivalence with the method described within this International Standard can be documented, alternative methods can be used for analysis.