为了响应美国环境保护局（USEPA）和受监管行业对更好的病原体鉴定方法的需求， 为了让实验室进行更有意义的定量质量控制（QC），研究人员开发了五种最新的方法 美国环保局支持的新法规通过实验室间验证进行了标准化和特征化 涉及方法性能定量评估的研究。这些方法包括: 美国环境保护局方法1601:两步法在水中检测雄性特异性（F+）和体细胞大肠杆菌噬菌体 浓缩程序； 美国环保局方法1602:单琼脂层法测定水中雄性特异性（F+）和体细胞大肠杆菌噬菌体 （三）程序； 美国环保局方法1605:氨苄西林膜过滤法测定成品水中的气单胞菌- 含万古霉素的糊精琼脂（ADA-V）； 美国环保局方法1623:通过过滤/IMS/FA在水中检测隐孢子虫和贾第虫；和 美国环保局方法1622:通过过滤/IMS/FA在水中发现隐孢子虫。 为这些方法进行的研究的目的是:确定实验室间和实验室间的特征 实验室内方法精度和偏差，使美国环保局和受监管行业 确定这些方法是否能满足预期的监管监管需求；建立 定量QC验收标准，因此实验室数据可以根据实际数据质量进行评估 期望。

In response to a need for better pathogen method characterization by both the US Environmental Protection Agency (USEPA) and the regulated industry, and for more meaningful quantitative quality control (QC) by laboratories, five of the most recent methods developed by USEPA to support new regulations were standardized and characterized through interlaboratory validation studies involving quantitative assessments of method performance. These methods include: USEPA Method 1601: Male-specific (F+) and Somatic Coliphage in Water by Two-step Enrichment Procedure; USEPA Method 1602: Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure; USEPA Method 1605: Aeromonas in Finished Water by Membrane Filtration using Ampicillin- Dextrin Agar with Vancomycin (ADA-V); USEPA Method 1623: Cryptosporidium and Giardia in Water by Filtration/IMS/FA; and, USEPA Method 1622: Cryptosporidium in Water by Filtration/IMS/FA. The objectives of the studies conducted for these methods were to: characterize interlaboratory and intralaboratory method precision and bias to enable USEPA and the regulated industry to determine whether the methods would meet anticipated regulatory monitoring needs; and, establish quantitative QC acceptance criteria, so laboratory data could be evaluated against realistic data quality expectations.