目前，确定在水处理厂中接种的微小隐孢子虫的实际灭活效率并不可行，这主要是因为目前的生存能力评估技术在天然水体中该病原体卵囊出现的低水平下不起作用。对微小隐球菌使用替代指标有可能帮助克服这一僵局。生物和非生物指示剂都被提议用作微小隐孢子虫的替代物。微小隐孢子虫很有希望的生物替代指标是需氧孢子形成菌。尤其是枯草芽孢杆菌孢子是很好的候选者，因为它们不是细菌- 致病性，相对容易培养和评估生存能力。本研究的目的是研究在水处理设施中发现的pH、温度和浓度条件下，使用两种化学消毒剂，即分子臭氧和一氯胺，枯草芽孢杆菌孢子的失活动力学与微小隐球菌卵囊的失活动力学是否准确匹配。包括9个参考文献、图表。

Determining the actual inactivation efficiency of Cryptosporidium parvumachieved in water treatment plants is not currently feasible due largely to the fact that current viability assessment techniques do not work at the low levels at which oocysts of this pathogen occur in natural waters. The use of surrogate indicators for C. parvum has the potential to help overcome this impasse. Both biological and non-biological indicators have been proposed for use as a surrogate for C. parvum. Promising biological surrogate indicators for C. parvum are aerobic spore-forming bacteria. In particular, Bacillus subtilis spores are good candidates because they are non-pathogenic and relatively easy to culture and assess for viability. The objective of this study was to investigate how accurately the inactivation kinetics of Bacillus subtilis spores matched those of C. parvum oocysts using two chemical disinfectants, molecular ozone and monochloramine, independently and sequentially under pH, temperature, and concentration conditions found in water treatment facilities. Includes 9 references, figures.