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Genomics informatics — Reliability assessment criteria for high-throughput gene-expression data 基因组信息学.高通量基因表达数据的可靠性评估标准
发布日期: 2021-10-25
本文件规定了高通量基因表达数据的可靠性评估标准。 它适用于评估从微阵列、下一代测序和其他形式的高通量技术生成的基因表达数据的准确性、再现性和可比性。 本文件确定了下一代RNA测序(RNA seq)过程的质量相关数据。本文件涵盖的测序平台仅限于短读测序器。RNA-seq用于突变检测和病毒识别不在本文件范围内。 本文件适用于人类健康相关物种,如人类、细胞系和临床前动物。其他生物物种不在本文件范围内。 从生物学角度来看,包括基因、转录本、亚型、外显子和连接在内的所有基因序列的表达谱都在本文的范围内
This document specifies reliability assessment criteria for high-throughput gene-expression data. It is applicable to assessing the accuracy, reproducibility, and comparability of gene-expression data that are generated from microarray, next-generation sequencing, and other forms of high-throughput technologies. This document identifies the quality-related data for the process of the next-generation sequencing of RNA (RNA-seq). The sequencing platform covered by this document is limited to short-read sequencers. The use of RNA-seq for mutation detection and virus identification is outside of the scope of this document. This document is applicable to human health associated species such as human, cell lines, and preclinical animals. Other biological species are outside the scope of this document. From a biological point of view, expression profiles of all genetic sequences including genes, transcripts, isoforms, exons, and junctions are within the scope of this document
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归口单位: ISO/TC 215/SC 1
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