本文件规定了测定饲料或饲料中可可碱的试验方法 工作范围为27 mg/kg至307 mg/kg的复合饲料，使用液相色谱法结合 紫外检测器（HPLC-UV）或使用液相色谱法在49 mg/kg至307 mg/kg的工作范围内 采用串联质谱法（LC-MS/MS）。 该方法已通过成年狗的补充复合饲料和马的补充复合饲料得到充分验证。 该方法也被认为适用于使用以下两种方法测定烘焙巧克力中的可可碱: HPLC-UV或LC-MS/MS系统。 如果扩展范围得到验证，则可以扩展工作范围。购买本文件时可获得的所有当前修订均包含在购买本文件中。

This document specifies a test method for the determination of theobromine in feed material or compound feed in the working range 27 mg/kg to 307 mg/kg using liquid chromatography coupled to a UV detector (HPLC-UV) or in the working range 49 mg/kg to 307 mg/kg using liquid chromatography with tandem mass spectrometry (LC-MS/MS). This method has been fully validated using complementary compound feed for adult dogs and complementary compound feed for horses. This method is also considered applicable for determining theobromine in baking chocolate using either HPLC-UV or LC-MS/MS systems. The working range can be extended provided the extended range is validated.All current amendments available at time of purchase are included with the purchase of this document.