ISO 14892IDF 177:2002规定了测定含至少10 μ g的供试品中维生素D的方法通过使用高效液相色谱法（HPLC）测定每100克维生素D【等于每100克400国际单位（IU）的维生素D】。 只有在测定维生素D3的情况下，测试样品仅含有维生素D3而不含有维生素D2(将作为内标物添加)，并且在测定维生素D2的情况下，测试样品仅含有维生素D2而不含有维生素D3(将作为内标物添加)，测定结果才是可靠的。这将通过不添加内标（维生素D2）的方法进行验证。

ISO 14892|IDF 177:2002 specifies a method for the determination of vitamin D in a test sample containing at least 10 μg of vitamin D per 100 g [equal to 400 International Units (IU) of vitamin D per 100 g] by using high performance liquid chromatography (HPLC). The results of the determination are only reliable if, in the case of determination of vitamin D₃, the test sample contains only vitamin D₃, and no vitamin D₂ (which will be added as an internal standard) and, in the case of determination of vitamin D₂, the test sample contains only vitamin D₂ and no vitamin D₃ (which will be added as an internal standard). This is to be verified by the procedure carried out without the addition of the internal standard (vitamin D₂).