1.1 本规程涵盖了工作场所空气中多分散金属加工液气溶胶中细菌内毒素浓度的个人采样和测定的定量方法。用户应具备微生物技术和内毒素检测的基本知识。 1.2 本规程的使用者可以在工作场所空气中短期或全班获得金属加工液气溶胶中内毒素的个人或区域暴露数据。 1.3 本规程给出了采样大气中内毒素浓度的估计值。 1.4 这种做法旨在尽量减少实验室间的差异，但不能确保结果的一致性。 1.5 预计该实践将通过提供内毒素采样、提取和分析方法的基础，促进来自金属加工流体大气，特别是金属去除流体大气的气载内毒素数据的实验室间比较。 1.6 1997年，职业安全与健康管理局（OSHA）成立了一个标准咨询委员会，就管理部门可以采取的措施向管理部门提出建议，以改善接触金属加工液的工人的健康。1999年7月，向OSHA劳工部助理部长提交了一份报告。小组委员会E34.50认为，如果开发一种标准方法，在使用水溶性金属去除液的设施中，基于LAL测定或其他可用的内毒素检测技术，为社区提供采样和分析个人气载内毒素暴露评估方法的指导，则用户群体将受益匪浅。 1. 7. 本规程并不试图设定或暗示工作场所环境中金属加工液气溶胶中内毒素的个人接触限值。 1.8 单位- 以国际单位制表示的数值应视为标准值。本标准不包括其他计量单位。 1.9 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.10 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 金属加工液气溶胶中的内毒素对吸入金属加工液气溶胶的工人存在潜在的呼吸道健康危害。因此，需要一个共识标准来提供有关使用金属加工液的工作场所空气中内毒素浓度的可靠数据。 5.2 这种测量金属加工流体大气中空气中内毒素浓度的做法将有助于更好地理解内毒素暴露-反应关系。 5.3 这种做法有助于比较方法和现场调查研究的实验室间数据。

1.1 This practice covers quantitative methods for the personal sampling and determination of bacterial endotoxin concentrations in poly-disperse metalworking fluid aerosols in workplace atmospheres. Users should have fundamental knowledge of microbiological techniques and endotoxin testing. 1.2 Users of this practice may obtain personal or area exposure data of endotoxin in metalworking fluid aerosols, either on a short-term or full-shift basis in workplace atmospheres. 1.3 This practice gives an estimate of the endotoxin concentration of the sampled atmosphere. 1.4 This practice seeks to minimize interlaboratory variation but does not ensure uniformity of results. 1.5 It is anticipated that this practice will facilitate interlaboratory comparisons of airborne endotoxin data from metalworking fluid atmospheres, particularly metal removal fluid atmospheres, by providing a basis for endotoxin sampling, extraction, and analytical methods. 1.6 In 1997, the Occupational Safety and Health Administration (OSHA) empanelled a Standards Advisory Committee to make recommendations to the Administration regarding measures that the Administration could take to improve the health of workers exposed to metalworking fluids. A report to the Assistant Secretary of Labor for OSHA was submitted in July 1999. Subcommittee E34.50 believes that the user community would benefit significantly if a standard method was developed to give the community guidance on a methodology for the sampling and analysis of personal airborne endotoxin exposure assessments in facilities using water-miscible metal removal fluids, based on the LAL assay or other endotoxin detection technologies as they become available. 1.7 This practice does not attempt to set or imply limits for personal exposure to endotoxin in metalworking fluid aerosols in workplace environments. 1.8 Units— The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.9 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.10 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 Endotoxins in metalworking fluid aerosols present potential respiratory health hazards to workers who inhale them. Therefore, a consensus standard is needed to provide reliable data on workplace airborne endotoxin concentrations where metalworking fluids are used. 5.2 This practice for measuring airborne endotoxin concentrations in metalworking fluid atmospheres will help to foster a better understanding of endotoxin exposure-response relationships. 5.3 This practice facilitates comparisons of interlaboratory data from methods and field investigative studies.