这项工作的目的是开发一种检测肠道病毒的方法，并评估新系统对环境水样的适用性。用于该分析的引物和探针是使用PE Applied Biosystems的Primer Express软件设计的。使用不同的RNA储备生成标准曲线。纯化后，通过紫外光谱对核酸进行定量。使用7700序列检测器扩增每个RNA储备的稀释系列，并计算标准曲线。每条曲线的R平方值大于0.99，且一致且可重复。该方法已成功用于定量细胞培养中的病毒扩增，并测量地下水浓缩物中碎片对PCR的抑制作用。 包括表格，如图所示。

The objective of this work was to develop an assay for detection of enteroviruses and evaluate the applicability of the new system for environmental water samples. Primers and probe used for this assay were designed using the Primer Express software from PE Applied Biosystems. Standard curves were generated using different RNA stocks. Following purification, the nucleic acid was quantitated by UV spectroscopy. A dilution series of each RNA stock was amplified using the 7700 Sequence Detector and standard curves calculated. The R squared value for each curve was greater than 0.99 and found to be consistent and reproducible. This method has been successfully used to quantitate virus amplification in cell culture and to measure the PCR inhibition caused by debris in groundwater concentrates. Includes tables, figure.