全球人口爆炸性增长和淡水资源减少 现在，我们已经在世界许多地方将再生水的使用作为一种惯例。品种 废水中存在大量微生物病原体，可在再生水中检测到。这个 本研究的目的是评估再生水通过时的水质变化 从处理厂到使用点的再生水分配系统 指病原体的发生。实验方法包括评估治疗效果 每月从三个不同的处理设施采集五个月的再生水样本。 然而，目前只有一个系统参与了抽样活动，另外两个系统参与了抽样活动 系统计划于2004年10月参与。处理设施位于三个区域 在一个大的公寓里，不同的州和重复使用的水用于冲厕和冷却塔 建筑群、购物中心和国家体育场。治疗方法包括生物治疗， 膜过滤、紫外线、臭氧和氯消毒。样本是 分析总大肠菌群和粪便大肠菌群、大肠杆菌、大肠杆菌O157、假单胞菌、， 气单胞菌、军团菌、HPC、体细胞和男性- 特异性大肠杆菌噬菌体，肠道病毒（肠道病毒， 通过RT-PCR检测甲型肝炎、诺沃克病毒和轮状病毒）核酸，贾第虫囊肿，传染性 隐孢子虫卵囊和理化水质参数。采集了水样 总大肠菌群和粪便大肠菌群、大肠杆菌和假单胞菌呈低水平阳性，表明 再生可能发生在分配系统中。还有一个样本对大肠杆菌呈阳性 O157表示可能需要额外消毒以确保重复用水的安全。包括10个参考文献和表格。

Explosive population growth and diminishing freshwater resources around the globe have now made reclaimed water use an established practice in many parts of the world. Varieties of microbial pathogens are present in wastewater and can be detected in reclaimed water. The objective of this study was to evaluate changes in quality of reclaimed water as it passes through the reclaimed water distribution systems, from treatment plant to point of use, with particular reference to pathogen occurrence. The experimental approach includes evaluating treated reclaimed water samples monthly for five months from three different treatment facilities. However, currently only one system is participating in the sampling campaign and the other two systems are scheduled to participate in October 2004. The treatment facilities are located in three different states and reuse water for toilet flushing and cooling towers in a large apartment complex, shopping mall and a national stadium. The treatments include biological treatment, membrane filtration, ultraviolet light, ozone and chlorine disinfection. The samples were analyzed for the presence of total and fecal coliforms, E. coli, E.coli O157, Pseudomonas, Aeromonas, Legionella, HPC, somatic and male-specific coliphage, enteric virus (enterovirus, hepatitis A, norwalk virus, and rotavirus) nucleic acid by RT-PCR, Giardia cysts, infectious Cryptosporidium oocysts, and physiochemical water quality parameters. Water samples were positive for low levels of total and fecal coliforms, E. coli, and Pseudomonas, suggesting that regrowth may be occurring in the distribution system. Also one sample was positive for E. coli O157 indicating that additional disinfection may be necessary to ensure the safety of reuse water. Includes 10 references, tables.