1.1 该方法旨在（定量）评估附着在合成聚合物材料和聚合物涂层的平面二维表面上的细菌数量，这些材料和涂层可能含有或不含有结合或结合的抗粘附剂。该方法侧重于评估表面减少细菌附着的能力。其他微生物如酵母和真菌分生孢子可使用该方法进行测试。 1.2 该测试方法定量地确定了允许细菌粘附的合成聚合物表面与不允许细菌粘附表面之间的细菌粘附差异，将从对照表面回收的生物数量与接触时间后从试样表面回收的数量进行比较。 这些程序需要掌握微生物学技术。 1.3 本试验方法规定了测量合成聚合物材料抵抗特定生物体粘附能力的适当方法。由于个体敏感性，一种测试生物体的结果可能不适用于其他生物体。 1.4 该测试方法旨在测量与已知在特定测试条件下支持细菌粘附的聚酯控制面板相比，无孔表面抵抗细菌粘附的潜在能力。 1.5 在该方法中，经抗菌处理的非多孔表面可能显示出抵抗细菌粘附的能力。该方法不旨在区分抗粘附性和抗微生物活性，也不旨在反映特定的最终用途或环境条件。应使用单独的测试技术（如测试方法）测量任何证明有能力抵抗细菌粘附的产品的抗微生物活性 第2180页 或ISO 22196。 1.6 该方法侧重于评估各种基材上的合成聚合物材料和聚合物涂层减少细菌附着的能力。 具有吸收或粘附表面的样本可能无法在测试前进行适当消毒，或可能在恢复过程中捕获接种的生物体，从而导致错误结果。该方法不适用于具有吸收性或粘性表面的试样。 1.7 以国际单位制表示的值应视为标准值。SI单位后括号中给出的值仅供参考，不视为标准值。 1.8 本标准并不旨在解决与其使用相关的所有安全问题（如有）。 本标准的使用者有责任在使用前建立适当的安全、健康和环境实践，并确定监管限制的适用性。 1.9 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《国际标准、指南和建议制定原则决定》中确立的国际公认标准化原则制定的。 =====意义和用途====== 5.1 该方法可用于评估合成聚合物材料和聚合物涂层中掺入或结合的抗粘附剂的有效性，以减少细菌附着于基材表面。 5.2 合成聚合物基材表面可随时间重复测试，以评估材料抵抗细菌粘附的持久能力。 5.3 该方法用于量化表面细菌定植的程度，以评估材料抵抗细菌粘附的能力，因为生物膜的形成会导致材料降解和故障。

1.1 This method is designed to evaluate (quantitatively) the number of bacteria attached to the flat, two-dimensional surfaces of synthetic polymeric materials and polymeric coatings on various substrates that may or may not contain bound or incorporated anti-adherent agents. The method focuses on assessing the ability of the surface to reduce bacterial attachment. Other microorganisms such as yeast and fungal conidia may be tested using this method. 1.2 This test method quantitatively determines the differences in bacterial adherence seen between synthetic polymeric surfaces that allow bacterial adherence and those that do not, comparing the number of organisms recovered from the control surface to the number recovered from the test specimen surface after the contact time. Knowledge of microbiological techniques is required for these procedures. 1.3 This test method specifies proper methods for measuring the ability of a synthetic polymeric material to resist adherence against specified organism. Due to individual sensitivities, the result of one test organism might not be applicable for other organisms. 1.4 This test method is designed to measure the potential ability to resist bacterial adherence of a non-porous surface compared directly to a polyester control panel known to support bacterial adherence under specific testing conditions. 1.5 Antimicrobial treated non-porous surfaces may demonstrate ability to resist bacterial adherence in this method. This method does not purport to differentiate between anti-adherence and antimicrobial activity nor is it designed to reflect specific end-use or environmental conditions. Any product that demonstrates ability to resist bacterial adherence in this method should be measured for antimicrobial activity using a separate test technique such as Test Method E2180 or ISO 22196. 1.6 The method focuses on assessing the ability of synthetic polymeric materials and polymeric coatings on various substrates to reduce bacterial attachment. The specimen with absorbing or adhesive surfaces may be unable to be disinfected properly before testing, or may trap inoculated organism during recovery process and thus lead to a false result. This method does not apply to specimens with absorbent or adhesive surfaces. 1.7 The values stated in SI units are to be regarded as standard. The values given in parentheses after SI units are provided for information only and are not considered standard. 1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 This method can be used to evaluate the effectiveness of incorporated or bound anti-adherent agents in synthetic polymeric materials and polymeric coatings intended to reduce the attachment of bacteria to the substrate surface. 5.2 The synthetic polymeric substrate surface may be tested repeatedly over time for assessment of persistent ability of a material to resist bacterial adherence. 5.3 This method is to quantify the degree of bacteria colonization of a surface to assess a materials ability to resist bacterial adherence because biofilm formation can contribute to material degradation and malfunction.