这项工作的目的是开发一种基于RT-PCR的低成本、高灵敏度系统，用于检测1升样本量内的病毒，用于对饮用水源的病毒污染进行常规监测。一种快速检测1型脊髓灰质炎病毒、MS2型脊髓灰质炎病毒和PRD-1型脊髓灰质炎病毒的方法已经开发出来，使用的是一种小型、低成本的1MDS胶囊过滤器。一公升的水以每分钟100毫升的速度通过过滤器。使用10至15毫升1.5%牛肉提取物溶液从过滤器中洗脱病毒。为了浓缩回收的病毒，使用高分子量截止旋转柱将过滤洗脱液的体积减小到150微升以下。 从浓缩物中提取脊髓灰质炎病毒RNA，并将其体积减小至20微升，一部分用于RT-PCR分析。样本收集、浓缩和提取方法可以在一公升水中检测到1 PFU的病毒。与目前使用的大容量方法相比，样品收集和洗脱在成本、时间、劳动力和每升灵敏度方面都有所改进。

The objective of this work was to develop a RT-PCR based low cost, highly sensitive system to detect viruses in a one liter sample volume, to be used in the routine monitoring of viral contamination of water sources intended for drinking. A rapid method for the detection of Poliovirus type 1, MS2, and PRD-1 has been developed using a small, low cost 1MDS capsule filter. One liter of water was passed through the filter at a rate of 100 mL per minute. Virus elution from the filter was accomplished using 10 to 15 mL of 1.5% beef extract solution. To concentrate recovered viruses, the filter eluent was reduced in volume to less than 150 uL using a high molecular weight cutoff spin column. Poliovirus RNA was extracted from the concentrates and reduced in volume to 20 uL and a portion used for RT-PCR assay. The sample collection, concentration, and extraction method can detect as little as 1 PFU of virus in one liter of water. The sample collection and elution is an improvement over the currently used large volume method in terms of cost, time, labor and sensitivity per liter.