ISO 22959:2009规定了用于测定食用油脂中多环芳烃（PAH）的高效液相色谱（HPLC）方法。 该方法已被验证为椰子，橄榄，向日葵和大豆油，并且可能适用于其他油，这取决于适当参数的确定。 PAHs的最低定量水平为0.1μg/ kg。可以与基线噪声区别的每个PAH的最低可能量尚未确定。方法的有效浓度范围为每个单独PAH为0.1μg/ kg至3,5μg/ kg。对于含有（轻）PAH含量>3,5μg/ kg的样品，可以使内容物进入有效范围进行稀释。也可以调整校准曲线的范围。然而，超过3,5μg/ kg的范围尚未得到验证。 可通过该方法测定的PAHs为:蒽，菲，荧蒽，芘，苯并[a]蒽，苯并[e]芘，苯并[a]芘，苝，苯并[ghi]二萘嵌苯，蒽醌，二苯并[蒽，蒽，茚并[1,2,3-cd]芘，苯并[a]荧蒽，苯并[b]荧蒽，苯并[k]荧蒽

ISO 22959:2009 specifies a high performance liquid chromatographic (HPLC) procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible fats and oils. The method has been validated for coconut, olive, sunflower, and soybean oil, and is possibly applicable to other oils, dependent on the determination of appropriate parameters. The lowest level of quantification for the PAHs is 0,1 µg/kg. The lowest possible amount of each PAH which can be distinguished from the baseline noise has not been determined. The validated concentration range of the method is 0,1 µg/kg to 3,5 µg/kg for each individual PAH. For samples containing (light) PAH contents > 3,5 µg/kg, dilution to bring the contents into the validated range is possible. It is also possible to adjust the range of the calibration curves. However, ranges exceeding 3,5 µg/kg have not been validated. PAHs which can be determined by this method are: anthracene, phenanthrene, fluoranthene, pyrene, chrysene, benzo[a]anthracene, benzo[e]pyrene, benzo[a]pyrene, perylene, benzo[ghi]perylene, anthanthrene, dibenzo[a,h]anthracene, coronene, indeno[1,2,3-cd]pyrene, benzo[a]fluoranthene, benzo[b]fluoranthene, benzo[k]fluoranthene.