1.1 本规程涵盖了水溶性金属加工液（MWF）中细菌内毒素浓度采样和测定的定量方法。 1.2 此实践的用户需要熟悉MWF的处理。 1.3 本规程给出了采样MWF中内毒素浓度的估计值。 1.4 这种做法取代了方法 E2250 . 1.5 这种做法旨在尽量减少内毒素数据的实验室间变化，但不能确保结果的一致性。 1.6 本标准并非旨在解决与其使用相关的所有安全问题（如有）。 本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.7 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 MWF中内毒素浓度的测定是一个参数，可用于审慎流体管理实践的决策（流体排放、清洁、充电或杀生物剂剂量）。 5.2 本标准为对水溶性MWF进行定量内毒素分析的分析员提供了实践。

1.1 This practice covers quantitative methods for the sampling and determination of bacterial endotoxin concentrations in water-miscible metalworking fluids (MWF). 1.2 Users of this practice need to be familiar with the handling of MWF. 1.3 This practice gives an estimate of the endotoxin concentration in the sampled MWF. 1.4 This practice replaces Method E2250 . 1.5 This practice seeks to minimize interlaboratory variation of endotoxin data but does not ensure uniformity of results. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 The determination of endotoxin concentrations in MWF is a parameter that can be used in decision-making for prudent fluid management practices (fluid draining, cleaning, recharging, or biocide dosages). 5.2 This standard provides a practice for analysts who perform quantitative endotoxin analyses of water-miscible MWF.