本文件包含细胞内细胞因子评估、主要组织相容性复合物多聚体定量、酶联免疫斑点技术和用于评估细胞增殖的羧荧光素琥珀酰亚胺酯追踪染料染色的方法。它还提供标本采集、运输和制备的基本方面；结果解释；以及质量保证和测试验证方法。

This document contains methods of intracellular cytokine evaluation, major histocompatibility complex multimer quantitation, enzyme-linked immunospot technology, and carboxyfluorescein succinimidyl ester tracking dye staining for the assessment of cellular proliferation. It also provides basic aspects of specimen collection, transport, and preparation; results interpretation; and quality assurance and test validation approaches.