本文件规定了使用形态学方法和/或分子方法检测和鉴定微藻、大型藻、蓝藻和迷宫菌的方法。 本文件中的形态学方法适用于从微藻、大型藻类、蓝藻和迷路菌中收获的湿生物质和干燥的未研磨生物质，这些微藻、大型藻类、蓝藻和迷路菌已生长和/或收获用于进一步加工和/或使用。 本文件中的分子方法适用于从微藻、大型藻类、蓝藻和迷路菌中收获的湿生物质和干燥和/或地面生物质，这些微藻、大型藻类、蓝藻和迷路菌已生长和/或收获用于进一步加工和/或使用。 本文件描述了一个工具箱，由几种识别方法组成，可根据识别的适用性和目的进行选择: -基于观察和参考分类学科学文献的形态学方法: -宏观识别； -光镜鉴定。 -序列测序和爆破的分子方法: -16srdna测序； -18srdna测序； -rbcL-DNA测序； -它的序列； -COX1基因测序； -凝灰岩基因测序。 本文件不涉及生物量的遗传纯度或已鉴定分类群的量化。 本文件不适用于具有高度降解DNA的高度加工生物量的分析，其中片段长度不足以扩增目标，且无法评估形态学特征。

This document specifies a method for the detection and identification of microalgae, macroalgae, cyanobacteria and Labyrinthulomycetes by using morphological methods and/or molecular methods. The morphological methods in this document are applicable to harvested wet biomass and to harvested dried unground biomass from microalgae, macroalgae, cyanobacteria and Labyrinthulomycetes that have been grown and/or harvested for further processing and/or use. The molecular methods in this document are applicable to harvested wet biomass and to harvested dried and/or ground biomass from microalgae, macroalgae, cyanobacteria and Labyrinthulomycetes that have been grown and/or harvested for further processing and/or use. This document describes a toolbox, consisting of several identification methods that can be chosen according to the applicability and purpose of the identification: - morphological methods based on observation and referring to scientific literature on taxonomy: - macroscopic identification; - light microscopic identification. - molecular methods for sequencing and blasting of sequences: - 16S rDNA sequencing; - 18S rDNA sequencing; - rbcL DNA sequencing; - ITS sequencing; - COX1 gene sequencing; - tufA gene sequencing. This document does not deal with genetic purity of the biomass or quantification of the identified taxa. This document is not suitable for the analysis of highly processed biomass with highly degraded DNA where the fragments’ length are not sufficient for amplification of the targets and the morphological characteristics cannot be assessed.