Experiments were conducted to evaluate the effects of UV irradiation on biological regrowth potential of two surface waters and two groundwaters. Biodegradable dissolved organic carbon (BDOC) differences between pre (before UV) and post (after UV) UV were evaluated. Rapid (5 day) assimilable organic carbon (AOC) measurements were used to ascertain a significant difference between pre and post-UV irradiated waters were also compared. Results suggested that UV may increase BDOC for some surface waters. A Student's t-test showed, with 90% confidence, that the BDOC for surface water A (SWA), increased after UV. The P17 AOC results for all the water showed, with greater than 95% confidence, the post-UV samples were greater than the pre-UV samples indicating an increase in regrowth potential after UV irradiation. Since an increase in biological regrowth potential was observed in some of the waters tested, utilities considering the addition of UV disinfection should carefully examine the regrowth potential of their source water(s) and distribution system water(s) before and after UV installation to determine effects and adjustments to secondary disinfection if required.