This powerpoint presentation begins by presenting a brief overview of
hollow fiber ultrafilters, including the mechanics of the hollow fiber
ultrafilter. Pictures are provided of the following topics: large volume sample in carboy;
gravity flow into 1L bottle;
pump driven flow;
pressure dampened and pressure measured;
sample pumped through the Ultrafilter; permeate flow exit;
and, return of concentrate to 1L bottle. Advantages of hollow fiber
ultrafiltration are given, along with reagents and solutions used for
ultrafiltration, validation experiments - indicator
organisms - STAG Grant; hollow fiber ultrafiltration process; Post-Ultrafiltration Detection Assays; validation study results; Cryptosporidium and Giardia Validation
Experiments - STAG Grant; Analysis of Retentate Post-
Filtration/Concentration; method comparison data; Bacillus subtilis Spore Recovery
Experiment; Development of Norovirus Assay Using
Hollow Fiber Ultrafiltration; and, results of initial analysis of MNV
using ultrafiltration + qPCR. Includes tables.