本文件旨在提供测试水果和蔬菜以确定储存期间腐败进展所需的所有程序和背景。这种测试方法是为了研究家用冷藏条件对水果和蔬菜货架期的影响而开发的（Pate和Brehm Stecher，2005）。说明从产品采购和样品制备开始，然后放置在环境室内。规定了整个实验过程中使用的数据收集程序，并制定了一份目测识别“变质”和“新鲜”物理属性的检查表。 微生物计数和数据收集的程序将逐步解释。引文:伊利诺伊州芝加哥ASHRAE Transactions第118卷第1部分

This document is intended to provide all procedures and background needed for testing fruits and vegetables for determination of spoilage progression during storage. This method of testing was developed for research into the effect of household refrigeration storage conditions on the shelf life of fruits and vegetables (Pate and Brehm-Stecher 2005). Instructions start with the procurement of the produce and sample preparation prior to placement within the environmental chamber. Data collection procedures used throughout the experiments are specified and a checklist for visually identifying physical attributes of "spoilage" and "freshness" are developed. Procedures for the counting of microorganisms and data collection are explained step-by-step.