ISO 11702:2016规定了通过酶学染色试验定量测定总甾醇含量的方法。该方法适用于动植物油脂，脂肪食品及相关产品中的游离酯化甾醇。该测定适用于1g至2g脂肪的样品量。 该方法不适用于深色油脂。该酶对胆固醇不是特异性的，而且还会氧化其他3-羟基固醇。该方法尚未对含有较高水平固醇的产品进行测试。 牛奶和奶制品（或来自牛奶和奶制品的脂肪）不包括在ISO 11702:2016的范围之内

ISO 11702:2016 specifies a method for the quantitative determination of the total sterols content by means of an enzymatic staining test. The method is applicable to free and esterified sterols in animal and vegetable fats and oils, fatty foods and related products. The determination is applicable to sample quantities of 1 g to 2 g of fat. The method is not applicable to dark coloured fats and oils. The enzyme is not specific for cholesterol, but also oxidizes other 3-hydroxysterols. The method has not been tested for products fortified with sterols at higher levels. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of ISO 11702:2016.