1.1 本试验方法涵盖了在受控实验室条件下暴露于接种物时，确定润滑剂或其成分在需氧水介质中的生物降解性程度的程序。本试验方法是一种最终生物降解试验，用于测量封闭式呼吸计中的需氧量。 1.2 本试验方法适用于评估挥发性和非挥发性润滑剂或润滑剂成分的生物降解。 1.3 本试验方法适用于在试验浓度下对试验微生物无毒且无抑制作用的润滑剂和润滑剂成分。 1.4 以国际单位制表示的数值应视为标准值。本标准不包括其他计量单位。 1.5 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 具体危害见第节 10 . 1.6 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 该试验方法的结果表明润滑剂或润滑剂成分的需氧水生生物降解程度。将试验材料暴露于受控实验室环境范围内的接种物后，测量耗氧量的速率和范围。在本试验中达到高度生物降解的试验材料可以假设在许多需氧水生环境中容易生物降解。 5.2 由于本试验方法的严格性，较低的结果并不一定意味着试验材料在环境条件下不可生物降解，但表明需要进一步试验以确定生物降解性。 5.3 如果测试结束时的pH值在6到8范围之外，并且测试材料的降解百分比小于50 %, 建议使用较低浓度的试验材料或较高浓度的缓冲溶液或两者同时重复试验。 5.4 为了验证接种物的活性，需要一种已知在本试验方法条件下可生物降解的参考或对照材料。如果标准物质的生物降解程度不超过60，则该试验必须被视为无效，并应使用新鲜接种物重复该试验 % ThO的 2. 28天内。 5.5 关于试验材料对接种物的毒性的信息可能有助于解释低生物降解结果。试验材料对接种物的毒性可通过在抑制控制系统中测试试验材料与参考材料的组合来评估。 如果包括抑制控制，如果参考材料的降解百分比低于40，则假设试验材料具有抑制作用 % （ISO 8192）。在这种情况下，建议用较低浓度的试验材料重复试验。 5.6 试验结束时，空白溶液中的总氧利用率超过60 mg O 2. /L使测试无效。 5.7 润滑剂或组分的水溶性或分散性可能会影响获得的结果，因此测试结果的比较可能仅限于具有类似溶解度的润滑剂或组分。 5.8 复杂混合物的行为并不总是与组分的个别属性一致。单个润滑剂成分的测试结果可能表明，含有这些成分的混合物（即完全配方的润滑剂）是否可生物降解，但应谨慎使用此类信息。

1.1 This test method covers a procedure for determining the degree of biodegradability of lubricants or their components in an aerobic aqueous medium on exposure to an inoculum under controlled laboratory conditions. This test method is an ultimate biodegradation test that measures oxygen demand in a closed respirometer. 1.2 This test method is suitable for evaluating the biodegradation of volatile as well as nonvolatile lubricants or lubricant components. 1.3 This test method is applicable to lubricants and lubricant components which are not toxic and not inhibitory to the test microorganisms at the test concentration. 1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazards are given in Section 10 . 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 Results from this test method suggest the degree of aerobic, aquatic biodegradation of a lubricant or lubricant component. The rate and extent of oxygen consumption is measured upon exposure of the test material to an inoculum within the confines of a controlled laboratory setting. Test materials which achieve a high degree of biodegradation in this test may be assumed to easily biodegrade in many aerobic aquatic environments. 5.2 Because of the stringency of this test method, low results do not necessarily mean that the test material is not biodegradable under environmental conditions, but indicate that further testing is necessary to establish biodegradability. 5.3 If the pH value at the end of the test is outside the range from 6 to 8 and if the percentage degradation of the test material is less than 50 %, it is advisable to repeat the test with a lower concentration of the test material or a higher concentration of the buffer solution, or both. 5.4 A reference or control material known to biodegrade under the conditions of this test method is necessary in order to verify the activity of the inoculum. The test must be regarded as invalid and shall be repeated using a fresh inoculum if the reference material does not demonstrate biodegradation to the extent of >60 % of the ThO 2 within 28 days. 5.5 Information on the toxicity of the test material to the inoculum may be useful in the interpretation of low biodegradation results. Toxicity of the test material to the inoculum may be evaluated by testing the test material in combination with the reference material in inhibition control systems. If an inhibition control is included, the test material is assumed to be inhibiting if the degradation percentage of the reference material is lower than 40 % (ISO 8192). In this case, it is advisable to repeat the test with lower concentrations of the test material. 5.6 Total oxygen utilization in the blank at the end of the test exceeding 60 mg O 2 /L invalidates the test. 5.7 The water solubility or dispersibility of the lubricant or component may influence the results obtained and hence comparison of test results may be limited to lubricants or components with similar solubilities. 5.8 The behaviors of complex mixtures are not always consistent with the individual properties of the components. Test results for individual lubricant components may be suggestive of whether a mixture containing these components (that is, fully formulated lubricants) is biodegradable, but such information should be used judiciously.