Prior to this research, water utilities needing to determine
the presence of Escherichia coli O157:H7 in their
raw water or drinking water needed to wait three to five
days for the results. This investigation was undertaken to
define methods that could achieve this in one working
day, which is necessary to allow utility staff to respond
in a timely manner and reduce the risk of infection to
water consumers.
On the basis of this research, two methods have been
identified that allow quick detection of very low levels of E.
coli O157:H7 in either raw or drinking water. This article
describes these procedures, which can dovetail into existing
Total Coliform Rule procedures or serve as stand-alone
methods. The procedures are quick (taking < 8 h) and user-friendly
enough that individuals with little technical skill can
accurately use these tests to obtain meaningful results. Includes 34 references, tables, figures.