该方法包括将试验部分溶解在氯仿中，用乙酸溶液从该溶液中提取游离甘油，用过量的高碘酸溶液氧化氯仿溶液中的1-单甘酯，添加碘化钾，并用硫代硫酸钠标准容量溶液滴定释放的碘。通过过量的高碘酸溶液氧化水溶液中的游离甘油，加入碘化钾，用硫代硫酸钠标准容量溶液滴定释放的碘。

The method consists in dissolving of a test portion in chloroform, extracting of free glycerol from this sulution with acetic acid solution, oxidizing of 1-monoglycerides in the chloroform solution by an excess of periodic acid solution, adding of potassium iodide and titration of the liberated iodine with a sodium thiosulfate standard volumetric solution. Oxidation of free glycerol in the aqueous solution by an excess of periodic acid solution, adding of potassium iodide titration of the liberated iodine with a sodium thiosulfate standard volumetric solution.