本文件提供了在实验室需氧条件下收集、处理和储存土壤以进行后续生物测试的标准程序。它适用于收集、处理和储存，以评估土壤对微生物、无脊椎动物（如生存、繁殖、生长、行为）和植物（如发育、生长）的影响。本文件不适用于需要始终保持厌氧条件的土壤处理。 本文件描述了如何最大限度地减少温度、含水量和氧气可用性差异对好氧过程的影响，以及土壤颗粒的分级，以促进可重复的实验室测定[1][2]。 本文件主要适用于温带土壤。 从极端气候（如永久冻土、热带土壤）收集的土壤可能需要特殊处理。 注:本文件未提供评估现场土壤生物群落结构和功能时土壤生物收集、处理和储存的标准程序。ISO 23611-1至ISO 23611-6中提供了此类标准程序。

This document provides standard procedures for the collection, handling and storage of soil for subsequent biological testing under aerobic conditions in the laboratory. It applies to the collection, handling and storage for assessing the effects of soil on microorganisms, invertebrates (e.g. survival, reproduction, growth, behaviour) and plants (e.g. development, growth). This document is not applicable to the handling of soil where anaerobic conditions need to be maintained throughout. This document describes how to minimize the effects of differences in temperature, water content, and availability of oxygen on aerobic processes as well as the fractionation of soil particles to facilitate reproducible laboratory determinations[1][2]. This document is mainly applicable to temperate soils. Soils collected from extreme climates (e.g. permafrost, tropical soils) can require special handling. NOTE This document does not provide standard procedures on the collection, handling and storage of soil organisms when assessing the structure and function of soil organism communities in the field. Such standard procedures are provided in ISO 23611？1 to ISO 23611？6.