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Statistical Analysis of Microbial Data Obtained by Serial Dilution - Agar Plate Methods 通过连续稀释-琼脂平板法获得的微生物数据的统计分析
发布日期: 2007-11-01
用于微生物计数的电镀技术仍然是重要的定量性能评估 饮用水合规性测试期间使用的工具(如常规大肠菌群分析) 以及实验室和中试规模的性能演示(如需氧孢子去除)。虽然 历史上,对这些方法的大量统计分析仅限于质量控制 方法开发、实验数据分析和实验室熟练程度也可以 通过正确分析数据中的可变性来增强。本文提出了一个简单的方法 评估方法框架- 膜法细菌定量中的相关误差 过滤-琼脂平板法,并对以下概率模型进行了初步讨论: 可用于量化平板计数数据中的不确定性。简单的回收实验 枯草芽孢杆菌孢子和大肠杆菌展示了这种情况的发生和发展 方法相关误差的量化。这种类型的方法误差分析对于 实验室、水供应商和工程师,尤其是在证明法规遵从性方面。包括11个参考文献和表格。
Plating techniques for microbial enumeration continue to be important quantitative performance assessment tools used during drinking water compliance testing (e.g. routine coliform analysis) and bench- and pilot-scale performance demonstrations (e.g. aerobic spore removal). While considerable statistical analysis of these methods historically has been limited to quality control and method development, the analysis of experimental data and laboratory proficiency can also be enhanced by the correct analysis of variability in the data. This paper presents a simple framework for assessing method-associated errors in the quantification of bacteria by membrane filtration - agar plate methods and provides a preliminary discussion of probabilistic models that may be used to quantify uncertainty in plate-count data. Simple recovery experiments for Bacillus subtilis spores and Escherichia coli are presented to demonstrate the occurrence and quantification of method-associated errors. This type of analysis of method errors is essential for laboratories, water suppliers, and engineers, particularly in demonstrating regulatory compliance. Includes 11 references, tables.
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发布单位或类别: 美国-美国给水工程协会
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